Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen

Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen

Blog Article

In the pandemic, rapid and accurate detection of SARS-CoV-2 is crucial in controlling the outbreak.Recent studies have shown a high detection rate using saliva/oral fluids as specimens for laboratory detection of the virus.We intended to evaluate the test performance of the Xpert Xpress SARS-CoV-2 cartridge assay in comparison to a conventional qRT-PCR testing, using saliva as biological specimen.Forty saliva samples from symptomatic participants were collected.

Conventional qRT-PCR revlon colorstay lip liner plum was performed for amplification of E and RdRp genes and the Xpert Xpress SARS-CoV-2 assay amplified E and N2 genes.In the conventional assay, the median cycle threshold value of the E gene was 34.9, and of the RdRp gene was 38.3.

In the Xpert Xpress assay, the median cycle threshold value of the E gene was 29.7, bolia outlet gent and of the N2 gene was 31.6.These results can allow a broaden use of molecular tests for management of COVID-19 pandemic, especially in resources-limited settings.